ReactomePA试用

安装补充包

• conda activate clusterprofiler
• ~/dev/xray/xray -c ~/etc/xui2.json &
• wget -e “https_proxy=http://127.0.0.1:20809“ https://github.com/YuLab-SMU/ReactomePA/archive/refs/heads/master.zip -O ReactomePA-master.zip
• BiocManager::install(“reactome.db”)
• devtools::install_local(‘ReactomePA-master.zip’)

读入数据

差异基因分析

library(stringr)
library(org.Hs.eg.db)
DEG <- subset(readRDS('DEG.rds'), !grepl('pseudogene', gene_type) & baseMean > quantile(baseMean)['25%'] & padj < 0.05)
rownames(DEG) <- t(as.data.frame(str_split(DEG$gene_id, '\\.')))[,1]
allEntrez = clusterProfiler::bitr(rownames(DEG), fromType="ENSEMBL", toType="ENTREZID", OrgDb=org.Hs.eg.db)
DEG$ENSEMBL <- rownames(DEG)
lfc <- merge(data.frame(DEG), allEntrez, by="ENSEMBL")
lfc <- lfc[order(lfc$log2FoldChange, decreasing=TRUE),]
geneList <- lfc$log2FoldChange
names(geneList) <- lfc$ENTREZID

x <- readRDS('DEG_filer.rds')
rownames(x) <- t(as.data.frame(str_split(x$gene_id, '\\.')))[,1]
cand.entrez = clusterProfiler::bitr(rownames(x), fromType="ENSEMBL", toType="ENTREZID", OrgDb=org.Hs.eg.db)$ENTREZID

进行分析

ORA

set.seed(123)
pway = ReactomePA::enrichPathway(gene = cand.entrez)
pway = clusterProfiler::setReadable(pway, OrgDb=org.Hs.eg.db)
pway = enrichplot::pairwise_termsim(pway)
pway@result

GSEA

set.seed(123)
pwayGSE <- ReactomePA::gsePathway(geneList, eps = 0)
pwayGSE = clusterProfiler::setReadable(pwayGSE, OrgDb=org.Hs.eg.db)
pwayGSE = enrichplot::pairwise_termsim(pwayGSE)
pwayGSE@result